Figure 2: Dynein processivity requires a single motor domain.
From: Tension on the linker gates the ATP-dependent release of dynein from microtubules
(a) Possible ring–ring communication between the two heads is abolished by replacing one of the monomers with a SRS-MTBD chimera. (b) A kymograph of SRS85:82/WT showing that it moves processively along MTs. (c) The average velocity (±s.d.) and run length (±95% CI) of the SRS-MTBD/WT constructs compared with WT/WT. (d) An example high-resolution tracking trace (black) and stepping fit (red) of the SRS85:82 head. (e) Histograms of the step sizes for the SRS (upper) and WT (lower) heads (mean±s.d.).
