Figure 5: T-HGK cKO mice develop insulin resistance mediated by IL-6 and IL-17.
(a,b) Fasting insulin (a) and glucose levels (b) in the sera of WT and T-HGK cKO mice. n, at least 18. (c) GTT tests were performed on 12-week-old WT and T-HGK cKO mice. Glucose levels were plotted versus time post glucose injection. n=6. (d) ITT tests were performed on 12-week-old mice. Blood glucose levels were measured at indicated time periods post insulin injection. n=6. (e,f) Immunoblotting analyses of p-IRS-1 (Y896 or S612), IRS-1, p-AKT, AKT or tubulin/actin in hepatocytes, skeletal muscle cells and adipocytes of 16-week-old (e) or 8-week-old (f) WT and T-HGK cKO mice. Mice were injected with 2 g kg−1 glucose for 1 h after 16-h fasting. WT, littermate controls (HGKf/f mice or CD4-Cre mice); HGK cKO, T-HGK cKO mice. *P-value<0.05; **P-value<0.01 (a–d, two-tailed Student’s t-test).
