Figure 2: MdfA-mediated Dq transport.
From: Export of a single drug molecule in two transport cycles by a multidrug efflux pump
(a) Characterization of Dq accumulation by E. coli UTL2 mdfA::kan overexpressing MdfA or its inactive mutant MdfA(R112M), or cells harbouring an empty plasmid. (b) Quantitative Dq accumulation. Cells were suspended in buffer containing glucose and Dq (1 μM), and allowed to accumulate Dq for 0 or 14 min. Dq fluorescence in these cells was determined after the cells were harvested and lysed, relieving their fluorescence quenching effect. Error bars indicate range of duplicate measurements. (c) Dq accumulation by control or MdfA-expressing cells in the presence of 0.8 mM CAM. (a,c) The experiments were repeated at least three times and the results shown are representative.
