Figure 4: Strategies for increasing the NADPH pool.
From: Metabolic engineering of Corynebacterium glutamicum for L-arginine production
(a) Changes in metabolic fluxes in AR1, AR3, AR4 and AR6 strains. Downregulation of the pgi gene (blue arrow) in the AR3 strain and the overexpression of the opcA, pgl, tal, tkt and zwf genes in an operon (red arrow) in the AR4 and AR6 strains led to the increased intracellular NADPH concentration. (b) The NADPH levels in AR1, AR3, AR4 and AR6 strains. The NADPH concentrations were measured from the cells obtained at the exponential growth phases during the 5 l fed-batch fermentation. Experiments were conducted in duplicates. Error bars indicate s.d.
