Table 1 Voltage-sensing properties of GEVIs.

From: Bright and fast multicoloured voltage reporters via electrochromic FRET

Fluorophore

HEK Cells

Neurons

 

Δ F/F per 100 mV (%)

Response to hyperpolarizing step

Response to depolarizing step

Δ F/F for single AP (%)

SNR for single AP

  

τ fast (ms)

τ slow (ms)

% fast

τ 1/2 (ms)

τ fast (ms)

τ slow (ms)

% fast

τ 1/2 (ms)

  

ECFP

−0.8

ND

ND

EGFP

−7.7

4.3

27

45

7.6

3.0

26

61

3.9

ND

Citrine

−13.1

4.8

21

38

7.5

3.1

21

62

3.8

9.7

8.8

mOrange2

−10.0

4.3

26

45

7.5

3.9

27

60

5.1

9.9

9.0

mRuby2

−8.7

4.3

27

65

4.7

3.6

20

65

3.8

5.4

7.2

mKate2

−4.5

2.8

35

25

9.2

4.0

25

44

6.7

ND

ArcLight Q239

−31.6

104

283

61

103

28

271

39

90

8.2

9.2

ASAP1

−28.8

3.0

29

67

3.5

2.3

39

88

1.9

17.8

13.1

QuasAr2

90

1.0

16

80

0.9

1.2

12

68

1.4

48

41

  1. AP, action potential; ECFP, enhanced cyan fluorescent protein; EGFP, enhanced green fluorescent protein; SNR, signal-to-noise ratio.
  2. Sensitivity, ΔF/F per 100 mV, was measured for a voltage step from −70 mV to +30 mV. Response times were characterized either by fast and slow time constants from a bi-exponential fit (τfast and τslow) or by τ1/2, the time to reach 50% of the steady-state response to a step of membrane voltage of duration at least 2 τslow. Half-response times are shorter for steps that do not reach steady state. Hyperpolarizing step: +30 mV to −70 mV; depolarizing step: −70 mV to +30 mV. All measurements were performed at 23 °C and represent the average of 6–9 cells. Illumination intensities were 3 W cm−2 for all reporters except for QuasAr2. Illumination intensity was 200 W cm−2 for QuasAr2. (ref. 23).
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