Figure 5: YAP inhibits transdifferentiation through DNp63 repression. | Nature Communications

Figure 5: YAP inhibits transdifferentiation through DNp63 repression.

From: YAP inhibits squamous transdifferentiation of Lkb1-deficient lung adenocarcinoma through ZEB2-dependent DNp63 repression

Figure 5

(a) Representative images showing haematoxylin and eosin and Yap, p63, Ck14 and Sp-c immunohistochemical staining on serial sections of KL mice tumours delivered with Lenti-U6-Cre or Lenti-shYap-Cre. Scale bar, 150 μm. (b,c) Ectopic YAP S127A expression downregulated DNp63 mRNA and protein levels in HTB-182 (b) and CRL-1848 (c) cells. (d,e) Knockdown of YAP upregulated DNp63 mRNA and protein levels in HTB-182 (d) and CRL-1848 (e) cells. (f,g) Ectopic expression of YAP 5SA but not YAP 5SA with C-terminal transactivation domain deletion dramatically decreased DNp63 mRNA and protein levels in HTB-182 (f) and CRL-1848 (g) cells. The asterisk represents the YAP 5SA-ΔC mutant form with its C-terminal transactivation domain deletion. Each sample was assayed in triplicates by quantitative real-time PCR. Representative data of real-time PCR and western blotting were shown from three independent experiments. Data are shown as mean±s.e.m. t-test, *P<0.05; **P<0.01; ***P<0.005.

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