Figure 5: Omomyc causes growth arrest and increases cell death in U87MG cells.

(a) Growth curves of U87MG infected with GFP control or GFP-Omomyc-expressing retroviruses. Total cell number was quantified at the indicated time points. Data are shown as the mean±s.e. of triplicates from a single experiment representative of three repeats. P-value at 3 days <0.05; P-value at 4 days <0.01, using a two-tailed t-test. (b) Cell death was assessed by Trypan blue staining. The number of dead cells was quantified at the indicated time points. Data are shown as mean±s.e. of triplicates. P-values were calculated by t-test. (c) U87MG cells were infected with a doxycycline-switchable lentiviral vector expressing Omomyc. Doxycycline treatment increases the number of β-galactosidase positive, senescent-like cells (left panels: typical β-galactosidase phase contrast images, right panel: quantification). The average of three experiments+s.d. is shown. P-values were calculated by a Student’s t-test.