Figure 7: Omomyc causes alterations in proteins linked to mitotic cell death.
From: Myc inhibition is effective against glioma and reveals a role for Myc in proficient mitosis
(a) Western blot analysis of samples from GBM cells shows that Omomyc causes a decrease in SAE1 expression in U87MG but not U373MG cells. GBM cells with a lentiviral Omomyc expression cassette were treated with doxycycline for 6 days to induce Omomyc expression. Two independent sets of samples are shown for each of the GBM cell lines. (b) Western blot analysis of GBM samples shows that Omomyc expression triggers an increase in PTP1B phosphorylation in U373MG cells. GBM cells with a lentiviral Omomyc expression cassette were treated with doxycycline for 6 days to induce Omomyc expression. A Taxol control was used to indicate the typical phosphorylation-induced mobility shift of PTP1B during mitotic catastrophe. Taxol-treated control samples are nonadjacent bands from the same western blot.
