Figure 7: Effects of hyperglycaemia on islet cell transcription factors and transporters.

(a–d) Representative examples of immunofluorescence staining for (red) Pdx-1 (a), Glut2 (b), MafA (c) or MafB (d) in a 4-week-diabetic βV59M islet. Insulin (green), glucagon (pink). (a) Insets show (upper) glu⁺ and (lower) ins⁺/glu⁺ cells that express Pdx1. (b) Inset shows an ins⁺/glu⁺ cell that expresses Glut2. (c) Inset shows an ins⁺/glu⁺ cell that does not express MafA. (d) Inset shows an ins⁺/glu⁺ cell that expresses MafB. Scale bars, 50 μm. (e) Pdx-1, Nkx6.1, MafA, Glut2, Arx, Pax6 and MafB mRNA levels assessed by qPCR in islets isolated from control mice (black bars) and 4-week-diabetic βV59M mice (white bars). Data are mean values±s.e.m., n=6–7 mice per genotype. (*P<0.05; Mann–Whitney test). (f) Nkx6.1, MafA, Glut2, Arx and MafB mRNA levels in FAC-sorted RFP⁺ cells isolated from control mice (black bars) and 4-week-diabetic βV59M mice (white bars). Data are mean values±s.e.m., n=4 mice per genotype. (*P<0.05; Mann–Whitney test).