Figure 4: PsIsc1 can replace the translocation domain of Avr1b. | Nature Communications

Figure 4: PsIsc1 can replace the translocation domain of Avr1b.

From: Unconventionally secreted effectors of two filamentous pathogens target plant salicylate biosynthesis

Figure 4

(a) PsIsc1 preferentially accumulates in haustoria. P. sojae-infected hyphae were observed using confocal microscopy at 10 hpi. P. sojae expressing Avr1b-mRFP showed specific haustorial accumulation (indicated by the arrow) of fluorescent Avr1b (red, upper panel), whereas PsIsc1-mRFP accumulated preferentially in haustoria (red, middle panel). P6497 expressing mRFP served as a control (lower panel). Scale bars, 20 μm. The arrow indicated haustoria were shown with tenfold magnification. (b) Structure of the fusion of full-length PsIsc1 and the C terminus of Avr1b (Avr1bCt: aa, 66–138, removing the signal peptide and RxLR-dEER domain). (c) Western blotting of proteins from P. sojae transformants expressing Avr1b-mRFP, Avr1bCt-mRFP and PsIsc1-Avr1bCt (PsIsc1-Avr1bCt1 and PsIsc1-Avr1bCt3). The P. sojae isolate P6497 served as a control for antibody specificity. (d) The phenotypes of hypocotyls from soybean cultivars HARO13 (Rps1b) and Williams (rps). Photographs were taken 2 dpi.

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