Figure 2: Regulation of various endothelial and vascular markers by Twist1.
From: Twist1 induces endothelial differentiation of tumour cells through the Jagged1-KLF4 axis
(a,b) Real-time PCR analysis of various endothelial markers including vWF, TIE2, CD105 and ICAM1 in Twist1-overexpressing OECM1 clones. Fold change of relative RNA expression was shown (bar graph). (c) Western blot analysis of CD105 and ICAM1 in Twist1-overexpressing OECM1 cell clones versus the control (cont.) clones. (d) Real-time PCR analysis of various vascular markers including EFNB2, NRP1 and EPHB4 in Twist1-overexpressing OECM1 clones. The OECM1 control clone was selected as the control group in panels a–d. Data shown in panels a,b,d are mean±s.d. of three independent experiments. Asterisk (*) indicates statistical significance (P<0.05 by Student’s t-test) between experimental and control clones.
