Figure 4: Jagged1 is essential for Twist1-induced endothelial differentiation.
From: Twist1 induces endothelial differentiation of tumour cells through the Jagged1-KLF4 axis
(a) Expression of CD144 in Twist1-overexpressing OECM1 cells with knockdown of Jagged1. Dot plots from flow cytometry and the percentage of cells expressing CD144 (bar graph) are shown. (b) 2.5-Dimensional (2.5D) tube-formation ability assays in Twist1-overexpressing OECM1 cells with knockdown of Jagged1. Images of tube formation and pixels representing tube-formation ability (bar graph) are shown. Scale bar, 50 μm. (c) Expression of various endothelial markers including vWF, TIE2, ICAM1 and CD105 in Twist1-overexpressing OECM1 cells. Fold change of the levels of various endothelial markers was shown. (d) Expression of EFNB2, NRP1 and EPHB4 in Twist1-overexpressing OECM1 cells with knockdown of Jagged1. (e) Expression of CD144 in GFP-labelled Twist1-overexpressing OECM1 cells with knockdown of Jagged1. Green: GFP; red: CD144; blue: DAPI. Scale bar, 50 μm. (f) Co-expression of Twist1 and CD31 in Twist1-overexpressing cells with knockdown of Jagged1. Green: hCD31; red: Twist1; blue: DAPI. Data shown in panels a–d are mean±s.d. of three independent experiments. Asterisk (*) indicates statistical significance (P<0.05 by Student’s t-test) between experimental and control (cont.) clones. The OECM1-Twist1 control clone was selected as the control group in panels a–f.
