Figure 7: Knockdown of KLF4 decreased stem-like property induced by Twist1.
From: Twist1 induces endothelial differentiation of tumour cells through the Jagged1-KLF4 axis
(a) Representative pictures of tumour sphere formation of various clones. Scale bar, 50 and 100 μm. (b) Analysis of tumour sphere-formation activity of various clones measured by primary, secondary and tertiary spheres. The number of tumour spheres formed after plating 1,000 cells was measured (bar graph). (c,d) The mRNA expression levels of two stemness genes, Wnt5A (c) and CCND2 (d), were measured in various clones using real-time PCR analysis (bar graph). Data shown in panels b–d are mean±s.d. of three independent experiments. Asterisk (*) indicates statistical significance (P<0.05 by Student’s t-test) between experimental and OECM1 control (cont.) clones.
