Figure 9: Additive tumour-killing effects of DAPT with cetuximab through abolishing the KLF4+ population and a model to summarize the discovery in this report.
From: Twist1 induces endothelial differentiation of tumour cells through the Jagged1-KLF4 axis
(a) Tumour-killing effects of DAPT and cetuximab using cell viability assays (the percentage of live cells after drug treatment; bar graph). Data shown are mean±s.d. of three independent experiments. Statistical significance (Student’s t-test) was indicated as * or ** between different groups. (b) Flow cytometry analysis of cell population staining with KLF4 and CD44 under different combinations of drug treatment. (c) Xenotransplantation experiments were performed to measure the tumour volume of mice injected with various drugs to determine the tumour-inhibiting effects of various drug treatment (single or combined). Data shown in panel c is mean±s.d. of five mice for each group. Statistical significance (Student’s t-test) was indicated as * or ** between different groups. (d) A model to depict the crucial role of the Jagged1-KLF4 axis to mediate endothelial differentiation and form tumour vasculature to support the metastatic process initiated by the induction of EMT by Twist1. cont, control.
