Figure 5: LPS enhances the association of MAIR-II with TLR4 and FcRγ chain, and amplifies Syk activation via FcRγ chain. | Nature Communications

Figure 5: LPS enhances the association of MAIR-II with TLR4 and FcRγ chain, and amplifies Syk activation via FcRγ chain.

From: Toll-like receptor 4 and MAIR-II/CLM-4/LMIR2 immunoreceptor regulate VLA-4-mediated inflammatory monocyte migration

Figure 5

(a,b,d,e,f) RAW264.7, its transfectants expressing Flag-tagged CD300a or Flag-tagged MAIR-II (a,b,d,e) and iMo (f) were left unstimulated or stimulated with LPS for 20 h. Cell lysates were immunoprecipitated with antibodies against TLR4 (a), Flag (b,d), DAP12 (e) or FcRγ chain (f), and immunoblotted with the antibodies indicated. (c) iMo were left unstimulated or stimulated with LPS for 20 h and analysed for Fcer1g and Tyrobp expression by using real-time reversre transcription–PCR. ***P<0.001 (two-tailed Student’s t-test). Error bars indicate s.d. (c). Data are representative of three (a) or two (b–e) independent experiments.

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