Figure 1: High-content screening identifies miRNAs regulating Salmonella infection. | Nature Communications

Figure 1: High-content screening identifies miRNAs regulating Salmonella infection.

From: Functional high-throughput screening identifies the miR-15 microRNA family as cellular restriction factors for Salmonella infection

Figure 1

(a) Schematic of screening strategy. (b) Percentage of Salmonella-infected cells following treatment with the library of miRNA mimics. Salmonella infection was performed at MOI 25 and analysed at 20 h.p.i. Average results from the three independent biological replicates of the screening are shown as fold change compared with the control miRNA cel-miR-231, in log2 scale. Results for 695 miRNAs are shown; 180 miRNAs decreased cell viability and were excluded. miRNAs highlighted in red and green increase or decrease infection by at least twofold, respectively. (c) Representative images of HeLa cells treated with cel-miR-231 (control), miR-15a and miR-363 (highlighted in b) and infected with Salmonella. Image analysis is shown in the bottom panel. Scale bar, 100 μm. (d) Heatmap representing the changes in Salmonella infection, at three time points (4, 8 and 20 h.p.i., MOI 25), upon treatment with the 17 miRNAs that decreased Salmonella infection by at least twofold in the screening. Results are based on quantification of the percentage of infected cells and are shown as fold change compared with control miRNA. The colour scale illustrates the relative infection (log2 scale).

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