Figure 2: Salmonella infection is inhibited by the miR-15 family. | Nature Communications

Figure 2: Salmonella infection is inhibited by the miR-15 family.

From: Functional high-throughput screening identifies the miR-15 microRNA family as cellular restriction factors for Salmonella infection

Figure 2

(a) Sequences of the mature forms of the human miR-15 family members. Mouse (mmu) and zebrafish (dre) miR-15a are shown for comparison. Seed sequence is highlighted. (b) Representative images of HeLa cells treated with members of the miR-15 family or control miRNA (cel-miR-231) and infected with Salmonella. Scale bar, 100 μm. (c) Percentage of HeLa cells infected with Salmonella wild-type or ΔSPI-2 strains, after treatment with members of the miR-15 family. (d) Percentage of HT-29 and (e) RAW 264.7 cells infected with Salmonella upon treatment with control miRNA, miR-15a or miR-16. (f) Representative images of HeLa cells treated with control miRNA, miR-15a or miR-16 and infected with Shigella. Scale bar, 100 μm. (g) Percentage of HeLa cells infected with Shigella, (h) DsRed expression quantified using qRT–PCR and (i) CFU quantification upon treatment with control miRNA, miR-15a or miR-16. Salmonella and Shigella infections were performed at MOI 25 (HeLa and HT-29) or MOI 10 (RAW 264.7) and analysed at 20 h.p.i. Results are normalized to control miRNA and shown as mean±s.e.m. *P<0.05, **P<0.01, ***P<0.001, two-tailed Student’s t-test; n≥3.

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