Figure 4: Cyclin D1 is required for Salmonella infection. | Nature Communications

Figure 4: Cyclin D1 is required for Salmonella infection.

From: Functional high-throughput screening identifies the miR-15 microRNA family as cellular restriction factors for Salmonella infection

Figure 4

(a) Overlap between genes downregulated upon overexpression of miR-15 family members (miR-15a, miR-16 and miR-503; average ≥1.5-fold) and genes upregulated by Salmonella infection (twofold or more; MOI 100). Functional categories enriched among the 71 overlapping genes are shown. Enrichment analysis was performed using IPA, applying Benjamini–Hochberg multiple testing correction; the eight most enriched categories are shown. Red line represents P=0.05. (b) Cyclin D1 expression determined using qRT–PCR upon overexpression of members of the miR-15 family. (c,d) Cyclin D1 expression determined using western blot upon infection with (c) Salmonella wild type at the indicated MOIs or (d) mutant strains defective in invasion (ΔSPI-1, MOI 100) or intracellular replication (ΔSPI-2, MOI 100). (e) Representative images of HeLa cells treated with control siRNA or cyclin D1 siRNA, and infected with Salmonella (MOI 25). Scale bar, 100 μm. (f) Percentage of HeLa cells infected with Salmonella, (g) GFP expression determined using qRT–PCR and (h) CFU quantification upon treatment with control siRNA or cyclin D1 siRNA. Salmonella infection was analysed at 20 h.p.i. Results are shown as mean±s.e.m., normalized to control miRNA (cel-miR-231; b) or control siRNA (f–h). *P<0.05, ***P<0.001, two-tailed Student’s t-test; n≥3.

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