Figure 4: 2DSA/GA-MC can distinguish between the shape and molecular weights of 10 nm and 30 nm fibres.
From: Solution-state conformation and stoichiometry of yeast Sir3 heterochromatin fibres
(a) Left panel, vHW analysis of a saturated nucleosomal array in low-salt Tris buffer and in 1 mM MgCl2 folding buffer. The extended sample is represented by closed blue circles, and the sample in folding buffer by open light blue circles. Middle and right panels, GA-MC plots of f/f0 versus molecular weight for extended and folded samples. (b) Same as in a, using samples prepared in phosphate buffer containing~40 mM Na+ (extended), and in this buffer supplemented with 8 mM MgCl2 (folded). (c) GA-MC data of samples in a and b using experimentally determined 
values (for examples, see Supplementary Fig. 7). Number in brackets is the expected molecular weight of a 12mer array, and numbers in parentheses are 95% confidence intervals.
