Figure 7: GBA1 deficiency increases the propagation of α-synuclein pathology in vivo.
From: Glucocerebrosidase depletion enhances cell-to-cell transmission of α-synuclein
(a) Immunohistochemistry of α-synuclein with three different antibodies. Boxed areas in the top panels are magnified in the panels below. Engrafted cells are indicated with arrowheads. Scale bars, top panel, 250 μm; lower panels, 20 μm. (b,c) α-Synuclein immunoreactivities quantified and expressed as a percentage of the grafted area; n=8, *P<0.01 by t-test. (d,e) Co-immunofluorescence analysis of engrafted cells (arrowheads). Pixel intensities of α-synuclein labels measured in 40 cells per animal (n=8). Scale bar, 5 μm. (f,g) Co-immunofluorescence analysis of engrafted cells stained for TH (arrowheads). Pixel intensities of α-synuclein labels measured in 40 cells per animal (n=6). Scale bar, 5 μm.
