Figure 7: Analysis of Sox2⁺ cells in normal and Pten-null prostates.

(a) Immunostaining of Wt AP prostate lobes with p63, CK8 and Sox2 antibodies. Pink arrowhead points to a basal cell, while yellow arrowhead points to a Sox2-positive luminal cell. (b) Immunostaining of p63-null UGS grafts with p63, CK8 and Sox2 antibodies. Note that no Sox2-expressing luminal cells are detected in p63-null prostates. (c) Quantification of the proportion of basal cells, Sox2-positive luminal cells and Sox2-negative luminal cells from AP lobes of Wt, Pten-mutant (9 weeks) and Pten-mutant (castrated for 5 weeks) prostates, respectively. Data are presented as mean±s.e.m. (d) Histological analysis of Sox2-expresing cells in a Wt prostate, a Pten-mutant prostate tumour and a castrated prostate tumour stained for Sox2 and p63. (e) Analysis of proliferative status of Sox2-positive cells in Wt, or Pten-mutant prostate sections stained for Sox2 and PCNA. Yellow arrowheads point to the double staining cells. Scale bars, 10 μm for a, b, d and 25 μm for e.