Figure 3: Analysis of RNAP noise-dependent intrinsic and extrinsic noise.
From: Contribution of RNA polymerase concentration variation to protein expression noise
(a) Random generation of cell distributions with a pre-determined mean and s.d. of the RNAP concentration from the total collection of cells. Left, total cell distributions obtained by combining all cells from Fig. 1c (~40,000 cells). Middle, distribution of the subset of cells (500 cells) randomly selected from the total cell distribution to satisfy a given gamma distribution with a pre-determined mean (μ) and s.d. (σ). Right, pairwise correlation between the CFP and mCherry intensities in the sub-sampled cells. (b–d) Intrinsic, extrinsic and total noise of the sub-sampled cells depending on RNAP noise. The dotted lines represent linear guidelines for clarity. Error bars indicate±s.d., n=1,000. (e) Contribution of RNAP noise to intrinsic and extrinsic noise. We compared the gene expression noises shown in Fig. 2c with those at an RNAP noise=0. The intrinsic and extrinsic noises at an RNAP noise=0 (open symbols) were obtained from the y axis intercepts resulting from linear fitting in b and c, respectively.
