Figure 4: Real-time observation of fluctuation in T7 RNAP concentration.
From: Contribution of RNA polymerase concentration variation to protein expression noise
(a) Time traces of YFP-T7 RNAP fluorescence intensity in single-cell lineages. The pink bold line follows a single lineage, and the other daughter cells have different light-coloured lines. Each open rectangle indicates cell division. (b) All time traces of T7 RNAP concentration (total 792 traces). The bold orange line represents the average intensity of YFP-T7 RNAP. (c) Analysis of the fluctuation in T7 RNAP concentration. After a steady-state condition was reached, we obtained the mean (μ) and s.d. (σ) of T7 RNAP intensity from 2 h of each time trace (inset). The histogram shows the s.d. of T7 RNAP intensity relative to the mean T7 RNAP intensity in a single time trace. The average relative s.d. (σ/μ) of T7 RNAP intensity in the time traces is 10±4%.
