Figure 5: Evaluation of the effect of the T7 RNAP concentration history on downstream protein noise at the single-cell lineage level.
From: Contribution of RNA polymerase concentration variation to protein expression noise
Together with T7 RNAP intensity, the intensities of CFP and mCherry were measured for ~5 h. We used 729 traces for the analysis but showed only 200 traces in a–c for clarity. Representative time traces of (a) CFP fluorescence intensity and (b) mCherry fluorescence intensity. The bold blue and red lines indicate the average. (c) Representative time traces of YFP-T7 RNAP fluorescence intensity. The integration times for YFP-T7 RNAP concentration of a single-cell lineage, 0, 30, 1 and 2 h from the reference point (320 min), are indicated. (d–f) Dependence of the intrinsic, extrinsic and total noise of the sub-sampled cells on RNAP noise. Here, we used the integrated YFP-T7 RNAP concentrations from Fig. 5c for the subsampling analysis, and the concentrations of CFP and mCherry of the selected cells measured at 320 min were used for noise analysis in a population. The dotted lines represent linear guidelines for clarity. Error bars indicate±s.d., n=1,000.
