Figure 4: Altered lipid composition in gABCG2-knockout parasites. | Nature Communications

Figure 4: Altered lipid composition in gABCG2-knockout parasites.

From: A female gametocyte-specific ABC transporter plays a role in lipid metabolism in the malaria parasite

Figure 4

(a) Lipid profile of gABCG2-disrupted trophozoite (ΔgABCG2-T) and stage IV gametocyte (ΔgABCG2-G) compared with wild type. T, trophozoite; G, gametocyte; PG, phosphatidylglycerol; PS, phosphatidylserine; Cer, ceramide. Means and ±s.d. are shown (n=3). ***P<0.001; **P<0.01; *P<0.05 (unpaired t-test). (b) Percentage contribution of lipid classes to total lipid composition of wild-type and ΔgABCG2 stage IV gametocytes. Neutral lipids are shown in blue, FC in green and polar lipids in red. (c) PCA of the neutral lipid classes DAG, TAG and CE. Left: score plot showing grouping of three replicate analyses of wild-type (red) and ΔgABCG2 (blue) samples. x (PCA1) and y (PCA2) axes represent the principal components accounting for the greatest and second greatest variability, respectively. PCA loading plot indicating the contribution of individual lipid species towards the observed differences in the lipid classes DAG, TAG and CE between wild-type and ΔgABCG2 gametocytes. For clarity reasons, only the three most changed lipid species are labeled. (d) Wild-type and gABCG2-disrupted gametocytes labelled with neutral lipid stain. BF, bright field. Scale bar, 5 μm.

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