Figure 8: The non-canonical motifs are required for 4E-BP-mediated eIF4E nuclear import.
From: 4E-BPs require non-canonical 4E-binding motifs and a lateral surface of eIF4E to repress translation
(a–x) S2 cells expressing HA-tagged versions of CUP (a–d; m–p), Thor (e–h; q–t) or 4E-T (i–l; u–x) or the corresponding 4E-BMs mutants (indicated on the left) were treated with LMB for 12 h (+ LMB) or with methanol as control (−LMB). The cells were fixed and the localization of the HA-tagged proteins and endogenous eIF4E was determined by indirect immunofluorescence using anti-HA and anti-eIF4E antibodies. The merged pictures show the HA signal in green and the eIF4E signal in red. Scale bar, 5 μm.
