Table 1 Thermodynamic parameters for the interaction of eIF4E with eIF4G and 4E-BP peptides.
From: 4E-BPs require non-canonical 4E-binding motifs and a lateral surface of eIF4E to repress translation
K D (M) | ΔH (kcal mol−1) | TΔS (kcal mol−1) | ΔG (kcal mol−1) | Molar ratio | |
|---|---|---|---|---|---|
Peptide+eIF4E | |||||
eIF4G 578-680 | 17±13 × 10−9 | −18.5±2 | 7.97 | −10.51 | 0.99±0.08 |
CUP C+NC | 9.1±0.5 × 10−9 | −34.5±0.35 | 23.86 | −10.78 | 1.05±0.02 |
CUP C | 1.6±0.1 × 10−7 | −16.84±0.04 | 7.70 | −9.13 | 1.01±0.01 |
CUP L+NC | 1.03±0.03 × 10−7 | −18.5±0.2 | 9.10 | −9.38 | 0.98±0.01 |
Thor C+NC | 1.4±0.3 × 10−9 | −16.8±1 | 4.90 | −11.87 | 0.95±0.02 |
Thor C | 2.26±0.06 × 10−6 | −12.1±2.8 | 2.24 | −9.82 | 1.06±0.01 |
Thor L+NC | nb | nb | nb | nb | nb |
4E-T C+NC | 5.6±2.4 × 10−9 | −22.8±3 | 11.73 | −11.11 | 0.95±0.01 |
4E-T C | 1.6±0.2 × 10−8 | −18.6±0.7 | 8.16 | −10.45 | 0.95±0.01 |
4E-T L+NC | nb | nb | nb | nb | nb |
Peptide+eIF4E (II-AA) | |||||
eIF4G 578-680 | 40±9.5 × 10−9 | −16.24±0.04 | 6.32 | −9.93 | 1.03±0.01 |
CUP C+NC | 5.0±0.8 × 10−8 | −18.6±0.7 | 8.79 | −9.84 | 0.98±0.01 |
Thor C+NC | 4.7±0.3 × 10−7 | −7.6±0.3 | −0.91 | −8.46 | 0.97±0.01 |
4E-T C+NC | 8.8±2 × 10−9 | −12.9±0.3 | 2.16 | −10.80 | 1.03±0.04 |
