Figure 2: Overall structure of the Ops*–ArrFL-1 complex.
From: Crystal structure of a common GPCR-binding interface for G protein and arrestin
(a) The active receptor Ops* (orange) and the ArrFL-1 peptide (purple) are shown in ribbon representation, and the ArrFL-1 side chains are shown as sticks. Oligosaccharides at Asn15NT (pink) and a palmitoyl chain at Cys3238.60 (black) are presented as sticks. The detergent molecule, β-D-octylglucopyranoside (blue), is shown within the ligand-binding pocket as sticks. (b) Side view and (c) top view of the binding crevice of Ops*. Major hydrogen bonding interactions of Ops* with ArrFL-1 are shown between Leu77 and Lys3118.48 from the NPxxY(x)5,6F motif (green), and Met75 and Arg1353.50 from the E(D)RY motif (blue). (d) Additional rotated top view of the binding crevice of Ops*. Hydrophobic interactions between ArrFL-1 and residues in the binding interface of Ops* protein moiety are shown as sticks and spheres (light-blue). Arg1353.50 of TM3, Val1383.53, Lys141CL2 of CL2, Glu2496.32, Val2506.33 of TM6 and Asn3108.47, Lys3118.48 of TM7/H8 are in van der Waals contact to ArrFL-1 (that is, separated by less than 4 Å).
