Figure 2: Wilms tumour DICER1 and DROSHA mutations impair pri- and pre-miRNA processing in vitro. | Nature Communications

Figure 2: Wilms tumour DICER1 and DROSHA mutations impair pri- and pre-miRNA processing in vitro.

From: Somatic mutations in DROSHA and DICER1 impair microRNA biogenesis through distinct mechanisms in Wilms tumours

Figure 2

(a) Schematic of pri-miRNA indicating RNase III cleavage sites. (b) DICER1 mutations affect pre-miRNA processing. Wild-type or mutant DICER1 was purified by immunoprecipitation and tested against pre-miRNA substrates. Arrowhead, mature miRNA; asterisk, processing intermediate formed by DICER1 RIIIA cleavage in the absence of RIIIB activity. (c) Mutations in the RNase III domains impair DROSHA activity. Wild-type or mutant DROSHA was purified by immunoprecipitation and tested against pri-miRNA substrates. Arrowhead, processed pre-miRNA.

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