Figure 5: FtsZ and DNA localization pattern in Eds cells.

Cell length, FtsZ fluorescence, DNA fluorescence and overlay of FtsZ (green) and DNA (red) fluorescence of 562 Eds cells are represented in a–d, respectively. Cells are sorted by increasing cell length from left to right that is, the leftmost bar represents the shortest and the rightmost bar the longest cell. Cell length ranges from 16.46 to 82.54 μm (mean 43.16±12.30). Dotted white line in b–d indicates the cell outline. (e–g) Phase contrast, FtsZ fluorescence, DNA fluorescence and FtsZ (green)/DNA (red) overlay images of immunostained Eds cells of different lengths displayed from left to right in e and f and top to bottom in g. h shows average fluorescence profiles of 301 cells with homogeneous FtsZ staining and non-segregated DNA (left), 189 cells with FtsZ accumulation at mid-cell and segregated DNA (middle), and 54 cells with FtsZ rings and segregated DNA (right). FtsZ fluorescence (green) and DNA fluorescence (red) expressed in arbitrary units (a.u.) are plotted along the cell length in percentage. LSCM images of a constricted immunostained Eds cell is shown in i, bright field and corresponding FtsZ fluorescence below. Inset shows an LSCM image of a FtsZ ring from a different cell. Scale bars, 10 μm in e and f, 20 μm in g and 2 μm in i.