Figure 3: FGF signalling imposes anterior neural plate fate in PCPs. | Nature Communications

Figure 3: FGF signalling imposes anterior neural plate fate in PCPs.

From: Fibroblast growth factor signalling controls nervous system patterning and pigment cell formation in Ciona intestinalis

Figure 3

Double fluorescent whole-mount in situ hybridization (WMISH) with tyramide staining for Ci-Six3/6 (red) and Ci-tyrp1/2a (green) at neurula stage of Ciona wild-type embryo (a) and at tailbud stage of Ciona electroporated embryos with ptyrp1/2a>LacZ (b), ptyrp1/2a>FGFRDN (c) and ptyrp1/2a>Ets:Vp16 (d) plasmids. Zoom on the expression domains, dashed line indicate the border among Ci-Six3/6 and Ci-tyrp1/2a-positive cells. Embryos showing separate channels for tyrp1/2a (green) that marks a10.97 derivatives (green asterisks) and very faintly a10.98s progenies (white asterisks) and Six3/6 (red) expression. On the right, schematic illustration of Ciona neural plate and neural tube cells at tailbud stage showing Ci-Six3/6 (red) and Ci-tyrp1/2a (green) expressing cells, adapted from Cole and Meinertzhagen, 2004. (a)Vegetal view, anterior is on the top; (b–d) lateral view, anterior is on the left. Scale bar, 50 μm; for zoomed embryos, scale bar, 20 μm; nuclear staining by DAPI (blue). Number (n) of embryos showing the observed phenotype out of the total counted embryos, referred to one experiment that was repeated at least three times. See also Supplementary Fig. 3.

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