Figure 1: Map-based cloning of the qPC1 QTL and grain protein content of transgenic plants in T1.
From: OsAAP6 functions as an important regulator of grain protein content and nutritional quality in rice
(a) Location of the qPC1 QTL on the genetic linkage map of chromosome 1. (b,c) Fine mapping of the qPC1 region using two mapping populations, with 6,000 and 4,008 plants, respectively. No. of Recs, number of recombinants between qPC1 and indicated molecular marker (b). Numbers in parentheses in c correspond to the numbers of recombinants in the b. (d) Genotypes and phenotypes of the recombinants. The phenotype of each recombinant was determined by progeny testing (Supplementary Table 2). (e) The OsAAP6 coding region of ZS97 was inserted into the vector pCAMBIA1301S under control of the CaMV 35S promoter to prepare the overexpression construct (OX). Arrows represent the direction of PCR primers. (f) The 1.8-kb promoter fragment and 5′-UTR of OsAAP6 from ZS97 with its own coding region was inserted into the vector pCAMBIA1301 to prepare the complementation construct (ZpZc). Arrows represent the direction of PCR primers. (g) The 580 bp cDNA fragment from the fourth exon of OsAAP6 was inserted into the dspCAMBIA1301 vector to generate the RNAi construct. Arrows represent the direction of PCR primers. (h) Grain protein contents of transgenic plants in T1. N, number of plants; (+) and (−) indicate transgene-positive and transgene-negative plants, respectively. P-values were produced by two-tailed t-test. Error bars, s.e.m.
