Figure 3: Natural variation of OsAAP6 in a 197 accession rice mini-core collection and GUS activities in transgenic plants driven by eight promoter fragments and 5′-UTRs. | Nature Communications

Figure 3: Natural variation of OsAAP6 in a 197 accession rice mini-core collection and GUS activities in transgenic plants driven by eight promoter fragments and 5′-UTRs.

From: OsAAP6 functions as an important regulator of grain protein content and nutritional quality in rice

Figure 3

(a) OsAAP6 gene structure and natural variation between alleles from ZS97 and NYZ. (b) Natural variation of OsAAP6 in 197 rice accessions of a mini-core collection compared with the NILs. (c) Cladogram of eight haplotypes. (d) Protein contents of brown rice in sub-populations A and B; raw data are provided in Supplementary Table 5; n, is the number of accessions. P-values were generated by two-tailed t-tests. Error bars, s.e.m. (e) OsAAP6 transcript levels in the endosperms of Sub1 cultivars with class A and B at 5 DAF; the number of accessions analysed is shown below each bar. The P-value was generated by a two-tailed t-test. Error bars, s.e.m. (f) Diagrams for the four deletions of the OsAAP6 promoter and 5′-UTR fused to the GUS gene. (g) Quantitative analysis of GUS activity in transgenic plants. Y and G indicate young panicles at 2 days before flowering and grains at 5 DAF, respectively. Data were from the transgenic lines planted in a randomized complete block design with three replications. P-values were produced by the Duncan test. Error bars denote s.e.m.

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