Figure 3: Gα12 induces translocation and activation of GEF-H1. | Nature Communications

Figure 3: Gα12 induces translocation and activation of GEF-H1.

From: Mechanistic insight into GPCR-mediated activation of the microtubule-associated RhoA exchange factor GEF-H1

Figure 3

(a) Induction of stress fibres by Gα12 requires GEF-H1. Wild-type or GEF-H1−/− MEFs ectopically expressing Gα12 were stained with anti-phalloidin or anti-Gα12 antibodies and imaged by confocal microscopy. Scale bar, 20 μm. (b) Gα12 expression triggers GEF-H1 relocalization and activation. GEF-H1−/− cells were transfected with GEF-H1–eGFP alone or together with a constitutively active Gα12 (GαQL) mutant or with GαQL and dominant-negative RGS peptide (rgRGS). Cells were stained with anti-phalloidin or anti-tubulin antibodies and imaged. Scale bar, 20 μm. (c) The correlation coefficient of GEF-H1–eGFP and polymerized microtubules co-localization in b was determined in 70 cells (or more) from three independent experiments. Error bars represent s.d. of at least three independent replicates, and P values derived from pairwise t-tests are indicated.

Back to article page