Figure 1: Characterization of S1PR1 in the hypothalamus of rats.
From: Hypothalamic S1P/S1PR1 axis controls energy homeostasis
Western blottings show (a) S1PR1 protein levels in different tissues of control rats (n=6). (b) S1PR1 protein levels in the arcuate (Arc), dorsomedial/vetromedial (DMH/VMH), PVN nucleus and in the lateral hypothalamus (LH) of control rats (n=6). Bars represent the mean±s.e.m. (c) Single and double immunostaining was performed to evaluate the co-localization of S1PR1 (green) with POMC, NPY and STAT3 (red) in the arcuate nuclei of rats, with × 50 magnification (left panel) or × 200 magnification (scale bar, 20 μm). (d) Western blotting shows the S1PR1 protein levels and STAT3 tyrosine phosphorylation in hypothalamic samples of rats at 12 h of fasting and after 6 h of refeeding (n=5). (e) A schematic representation showing the localization of S1P injection. S1P was injected into the VMH of rats (coordinates 2.3/3.3 mm posterior to the bregma, ±0.6 mm lateral to midline and 10.2 mm below the surface of the skull). (f) Single immunostaining was performed to evaluate STAT3 tyrosine phosphorylation (green) in the hypothalamus of rats 30 min after vehicle (left) or S1P 50 ng (right) injections in the same rat, with × 100 magnification (scale bar, 20 μm).
