Figure 4: Effect of S1PR1 siRNA on food consumption and energy expenditure.
From: Hypothalamic S1P/S1PR1 axis controls energy homeostasis
(a) A schematic representation showing the localization of S1PR1 siRNA injection into the MBH of mice (coordinates of 1.5 mm posterior to the bregma, 5.8 mm below the surface of the skull and 0.4 mm lateral to midline). (b) Time-course of S1PR1 protein levels in VMH/DMH after S1PR1 siRNA injection in mice. (c) S1PR1 protein levels in different brain regions. (d) Daily food consumption after S1PR1 siRNA injection in mice (n=6). (e) Thirty-six hours of cumulative food intake (n=6). (f) Western blotting shows the effects of S1PR1 siRNA on STAT3 phosphorylation in the hypothalamus in mice fed on chow diet (n=4). (g) Oxygen consumption, carbon dioxide production and respiratory exchange ratio during 24 h after S1PR1 siRNA injection (n=6). Data were expressed by using mean±s.e.m.*P<0.05 versus scramble (Student’s t-test).
