Figure 5: STAT3 controls S1PR1 protein levels in neurons.
From: Hypothalamic S1P/S1PR1 axis controls energy homeostasis
Western blottings show (a) S1PR1 protein levels in GT1-7 cells after leptin (40 μmol l−1) incubation, performed in three independent experiments. (b) Effect of STAT3 siRNA transfection on STAT3 protein levels in GT1-7 cells. (c) Effect of leptin (40 μmol l−1) incubation on S1PR1 protein levels in GT1-7 cells transfected with STAT3 siRNA. (d) Effect of leptin on S1PR1 protein levels in the hypothalamus of control rats (n=4). Hypothalamic samples were obtained 8 and 12 h after acute leptin (10−6 M) injection. (e) S1PR1 protein levels in control rats injected with saline (2 μl), leptin (10−6 M) or JSI124 plus leptin. Hypothalamic samples were obtained 8 h after leptin injection (n=4). (f) Evaluation of S1PR1 protein levels on the hypothalamus of ob/ob mice and (g) db/db mice, and C57BL6/J (n=4). Data were expressed by using mean±s.e.m. *P<0.05 versus vehicle and #P<0.05 versus wild-type (Student’s t-test).
