Figure 6: Defective S1PR1 expression in the hypothalamus of obese rodents.
From: Hypothalamic S1P/S1PR1 axis controls energy homeostasis
Western blottings show S1PR1 protein levels in the hypothalamus of (a) Wistar rats (n=8), (b) C57BL6/J mice (n=4) and (c) Swiss mice (n=4) after HFD treatment. (d) Hypothalamic S1PR1 mRNA levels in control and obese Wistar rats (n=10). (e) Determination of S1P serum levels in control and obese C57BL6/J mice (n=6–8). (f) Determination of food consumption after ICV injection of vehicle (dimethylsulphoxide (DMSO)), S1P (50 ng) and SEW2871 (50 nM) in obese rats (n=8). (g) Western blotting shows S1P- and SEW2871-induced STAT3 tyr705 phosphorylation in hypothalamic samples of obese Wistar rats (n=6). (h) POMC and NPY mRNA were examined using real-time PCR assay 9 h after ICV S1P (50 ng) injection (n=8). (i) Oxygen consumption/carbon dioxide production after single injection of S1P into the third ventricle of obese rats (n=5). (j) Locomotor activity (n=5). (k) Determination of food consumption after chronic ICV infusion of S1P 2.08 ng h−1 or vehicle 0.25 μl h−1 through the osmotic mini-pump during 9 days. (l) Total body weight and (m) epididymal fat pad weight after chronic delivery of S1P in the hypothalamus of obese rats (n=6). Data were expressed by using mean±s.e.m. #P<0.05 versus chow and *P<0.05 versus obese plus vehicle (Student’s t-test).
