Figure 3: The effect of TLE6 on early mouse embryogenesis.

(a) After hormonal stimulation and in vivo mating with normal males, embryos were flushed from the oviduct of control and Tle6^Null female mice at E0.5, E1.5, E2.5 and E3.5 and imaged by DIC. Scale bar, 50 μm. (b) The number (mean±s.e.m.) of embryos flushed from the oviducts of more than five controls and Tle6^Null female mice at E0.5, E1.5, E2.5 and E3.5. after hormonal stimulation and mating with normal male mice. (c) Immunoblot of egg (20) lysates from control and Tle6^Null female mice probed with antibodies to MATER, Filia, FLOPED, TLE6 and GAPDH. (d) The abundance (mean±s.e.m.) of the SCMC components in control and Tle6^Null eggs (20) was determined by immunoblot using four independent samples from each group and setting protein abundance at 100% for control eggs. (e) Localization of TLE6, MATER, FLOPED and FILIA in ovulated eggs from control and Tle6^Null females was determined by confocal microscopy and DIC. Phalloidin labelled with Alex Fluor 546 was used to detect F-actin. Scale bar, 20 μm.