Figure 4: CendR-mediated cellular uptake is regulated by nutrient availability.

(a) Network analysis of genome screen hits using IPA. Top pathways associated with genomic hits (Supplementary Data 1) and the corresponding P-values (calculated by IPA) are shown. (b) Regulation of R-Ag uptake by nutrient conditions and other stimuli. After the indicated treatments (x axis), PPC1 cells were incubated with R-Ag as described in Methods. The average intensity of internalized R-Ag per cell was normalized to cells cultured in complete media and is shown as relative uptake (y axis). (c) Stimulation of R-NA uptake by nutrient deprivation. After the indicated treatments (x axis), PPC1 cells were incubated with R-NA as described in Methods. The average intensity of internalized R-NA per cell was normalized to cells cultured in complete media as relative uptake (y axis). (d) R-Ag and other endocytic probes respond differently to environmental cues. After the indicated treatments, PPC1 cells were incubated with different endocytic probes (upper right panels) for internalization as described in Methods. The fluorescence intensity of each probe under the indicated conditions (x axis) was normalized to cells cultured in complete media as relative uptake (y axis). Error bars indicate s.e.m. (3–5 replicates). *P<0.05, **P<0.01 and ***P<0.001 (Student’s t-test) in comparison with complete media conditions of the corresponding probes.