Figure 5: CendR peptide initiates an NRP1-dependent endocytosis process resembling macropinocytosis.
From: An endocytosis pathway initiated through neuropilin-1 and regulated by nutrient availability
(a) PPC1 cells were cultured in complete, AA-free or glucose-free media for 16 h followed by the incubation with R-Au (dense dots, 50 nm) for 30 min and fixation. Representative TEM images at cell entry sites are shown. (b) Representative TEM images for intracellular organelles containing R-Au (50 nm) with characteristics of endosome and MVB. (c) NRP1 dependence of CendR endocytosis. HeLa cells without or with transfection of NRP1 expression plasmids (NRP1 WT or ΔSEA) were incubated with R-Au (dense dots, 50 nm) for 30 min before fixation. Representative TEM images are shown. Scale bar, 200 nm.
