Figure 1: SMBA1–3 directly bind to the Bax protein but not to other Bcl2 family members. | Nature Communications

Figure 1: SMBA1–3 directly bind to the Bax protein but not to other Bcl2 family members.

From: Small-molecule Bax agonists for cancer therapy

Figure 1

(a) Structural modelling of SMBA1–3 in the S184 binding pocket in the C terminus of Bax protein using the all-atom AMBER molecular mechanics force field. The PDB files of the modelled complex structures are shown by detailed all-atom views of the predicted interactions for all three compounds along with labels for each residue. (b) Fluorescent Bak BH3 domain peptide (3 nM) was incubated with purified 6 nM of human Bax protein or other Bcl2 family member(s) in the absence or presence of increasing concentrations (that is, 0–500 nM) of SMBA1, SMBA2 or SMBA3 in the binding affinity assay buffer. Binding affinities were analysed using a competition fluorescence polarization assay. The error bars indicate ±s.d. of three separate experiments.

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