Figure 3: SMBAs induce Bax insertion into mitochondrial membranes and Cyt c release. | Nature Communications

Figure 3: SMBAs induce Bax insertion into mitochondrial membranes and Cyt c release.

From: Small-molecule Bax agonists for cancer therapy

Figure 3

(a) A549 cells were treated with 5 μM of SMBA1, SMBA2 or SMBA3 for 24 h. Bax and Cyt c were analysed by immunofluorescence staining. Images were merged using Openlab 3.1.5 software. Areas of co-localization are yellow. Scale bar, 10 μm. (b) A549 cells were treated with increasing concentrations of SMBA1, SMBA2 or SMBA3 for 24 h. Mitochondria were isolated and alkali extraction of Bax was performed. The alkali-resistant (that is, non-extractable) Bax was determined by western blot using a Bax antibody (1:500 dilution). (c) A549 cells were treated with increasing concentrations of SMBA1, SMBA2 or SMBA3 for 24 h. Mitochondrial and cytosolic fractions were isolated. Levels of Cyt c in these two fractions were analysed by western blot (full blots can be found in Supplementary Fig. 7).

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