Figure 1: The oligomerization-deficient Dam1 complex retains an intact microtubule-binding site. | Nature Communications

Figure 1: The oligomerization-deficient Dam1 complex retains an intact microtubule-binding site.

From: Kinetochores require oligomerization of Dam1 complex to maintain microtubule attachments against tension and promote biorientation

Figure 1

(a) Kymographs show single molecules of GFP-tagged wild-type (top) and oligomerization-deficient (bottom) Dam1 complex as they bound to and diffused along taxol-stabilized microtubules. Scale bar applies to both kymographs; image brightness and contrast were adjusted identically. (b) Histograms of GFP brightness intensity for single molecules of wild-type (WT, black trace, n=717) and oligomerization-deficient (OD, green trace, n=472) Dam1 complex on microtubules. Dashed-blue line indicates the average height of single-step photobleaches under identical imaging conditions (n=295). Brightness values are reported as mean±s.d. (c) Residence time distributions for individual Dam1WT (n=717) and Dam1OD (n=564) complexes on microtubules. Mean residence time, τ±error of the mean was estimated by bootstrapping analysis (see Methods for additional details). (d) Mean-squared displacement (MSD) versus time lag for single particles (WT, n=654; OD, n=506) represented in c and d. Error bars on each point indicate s.e.m. Dashed lines indicate weighted linear fits to the data, yielding the diffusion constants, D±s.d. of the fit.

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