Figure 3: The Dam1 complex loads onto kinetochores in the absence of oligomerization. | Nature Communications

Figure 3: The Dam1 complex loads onto kinetochores in the absence of oligomerization.

From: Kinetochores require oligomerization of Dam1 complex to maintain microtubule attachments against tension and promote biorientation

Figure 3

(a) Survival versus force curves for beads coated with Ndc80 complex alone (black trace, n=26) or in the presence of free wild-type (WT, blue trace, n=60) or oligomerization-deficient (OD, red trace, n=43) Dam1 complex in solution. (b) As in a, using beads instead coated with purified kinetochore particles lacking functional endogenous Dam1 complex (dad1-1 kinetochores). Beads were assayed alone (black trace, n=42), and in the presence of purified, recombinant Dam1WT (blue trace, n=81) or Dam1OD (red trace, n=78) complex added free in solution.

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