Figure 1: Preliminary map of the Shigella flexneri needle architecture. | Nature Communications

Figure 1: Preliminary map of the Shigella flexneri needle architecture.

From: High-resolution structure of the Shigella type-III secretion needle by solid-state NMR and cryo-electron microscopy

Figure 1

(a) Anchor point identification in the absence of previous structural knowledge. Proximity anchors are identified by at least two unambiguous (corresponding to well-resolved and readily assigned cross-peaks) or abundant (≥4) contacts in spectra of [1-13C]-glucose- and [2-13C]-glucose-labelled MxiH needles (gray lines, line thickness proportional to the number of contacts). (b) Strategy for assignment of ssNMR constraints arising from distinct intra-molecular and inter-molecular interfaces in helical assemblies. Pink lines indicate interface assignments of a given contact that are inconsistent with the axial displacement among symmetry-related subunits, and green lines the correct interface assignments.

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