Figure 4: Long-range ssNMR constraints detected using two complementary isotopic labelling schemes. | Nature Communications

Figure 4: Long-range ssNMR constraints detected using two complementary isotopic labelling schemes.

From: High-resolution structure of the Shigella type-III secretion needle by solid-state NMR and cryo-electron microscopy

Figure 4

Chemical-shift unambiguous long-range constraints identified in (a) [1-13C]-glucose- and (c) [2-13C]-glucose-labelled samples. In a and c interactions are colour-coded according to the connecting subunits as follows: green and cyan: intra-subunit; blue, light blue and magenta: interface i, i±5 or i, i±6; orange and red: interface i, i±11. (b) Partition diagram of the ssNMR constraints identified in this study considering the different 13C-labelling schemes. (d) Residue–residue contact map of the ssNMR constraints, showing medium- to long-range correlations (|i−j|>2) present in the data.

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