Table 2 Labelled CK applied to cotyledons is transported to the root tip.

From: Shoot-derived cytokinins systemically regulate root nodulation

 

Control 1

Control 2

Control 3

Sample 1 (pmol per 1sample)

Sample 2 (pmol per 1sample)

Sample 3 (pmol per sample)

Labelled iP, iPR and iPRPs

ND

ND

ND

1.0448

0.6634

1.8270

Labelled tZ, tZR and tZRPs

ND

ND

ND

0.0237

0.0152

0.0341

Labelled cZ, tZR and cZRPs

ND

ND

ND

0.0081

0.0037

0.0066

  1. CK, cytokinin; ND, not detected.
  2. Labelled iPR, [13C10,15N5]N6-(Δ2-isopentyl) adenine riboside, was applied to cut edges of cotyledons (3-day-old seedlings). Root tips (2 mm) were collected 4 h after the treatment. Labelled and authentic CKs were analysed by mass spectrometry. The applied iPR is metabolized to iP, iPRPs, tZ-type CKs and cZ-type CKs. CK contents of untreated plants were determined as control. Data from three independent replicates are shown.
Back to article page