Figure 2: SC-specific knockout of LKB1 attenuates myelination during development. | Nature Communications

Figure 2: SC-specific knockout of LKB1 attenuates myelination during development.

From: Phosphorylation of LKB1/Par-4 establishes Schwann cell polarity to initiate and control myelin extent

Figure 2

(a) SC-specific deletion of LKB1 was accomplished by crossing the floxed-LKB1 mice with the CNP-Cre mice. (b) Sciatic nerves from a WT littermate and the SC-specific LKB1 KO mice. (c) Time course of sciatic nerve myelination in WT littermates and SC-specific LKB1 KOs at 7, 14 and 60 days postnatal with Luxol Fast Blue staining. Scale bar=10 μm. (d) Immunostaining for LKB1 (red) from the sciatic nerves in the WT littermate and the SC-specific LKB1 KO mice. Scale bar=10 μm. (e) Western blots of WT littermates and SC-specific LKB1 KO nerves at 14 days postnatal were probed with antibodies to LKB1, Oct-6, Krox20 and the myelin protein P0. β-actin serves as a loading control. Representative western blots and sciatic nerves are displayed from three knockout and three WT littermates.

Back to article page