Figure 3: Knockout of LKB1 delays the maturation of SCs and myelination.

(a,b) Immunostaining for Krox20 (green) illustrates the number of promyelinating and myelinating SCs along the sciatic nerve of a WT littermate and the SC-specific LKB1 KO at 14 days postnatal. (c,d) Immunostaining for Oct-6 (green) illustrates the number of premyelinating SCs. Scale bar=100 μm. (e,f) Quantification of the number of Oct-6−, Krox20−, Sox2−, S100β-positive SCs per mm² and number of MBP-positive myelin internodes per mm² in the WT littermate and the SC-specific LKB1 KO sciatic nerves at 14 days postnatal. Error bars represent s.d. Asterisks represent significance based on Student’s t-test as compared with the WT control (P<0.004). (g,h) Immunostaining for MBP (red) illustrates the myelin internodes in the sciatic nerve of a WT littermate and the SC-specific LKB1 KO at 14 days postnatal. Scale bar=100 μm. (i,j) S100β (green) illustrates the number of SCs along the sciatic nerve of a WT littermate and the SC-specific LKB1 KO at 14 days postnatal. Scale bar=100 μm. Nuclei are detected by DAPI (blue). Results are obtained from three knockout and three WT littermates.